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Virology Core - Core F


Core Director:
William Borkowsky, M.D.
Phone: 212-263-6426
Email: william.borkowsky@med.nyu.edu

Core Location:
Bellevue Hospital, New Bellevue - 8 W 51

Services and Prices:

  • HIV-1 p24 Ag - $25
  • HIV-1 acid-dissociated p24 Ag - $30
  • HIV-1 qualitative PBMC or plasma culture - $200
  • HIV-1 quantitative PBMC or plasma culture - $225
  • HIV-1 DNA PCR - $80
  • HIV-1 antibody ELISA - $50
  • HIV-1 Western Blot - $50
  • HIV-1 Heteroduplex assay - $125/specimen
  • HIV-1 RT resistance testing - $100
  • HIV-1 Pol resistance testing - $100
  • HIV RNA/DNA PCR - $100
  • serum or plasma processing and storing (-135C) - $15
  • PBMC separation and storing (-135C) - $35
  • HIV culture supernatant ministock.storage - $20
  • MT-2 Assay - $50
  • CMV culture - $100
  • CMV PCR - $100
  • HSV culture - $50
  • JC PCR - $100
  • HCV RNA/DNA PCR - $125

Training in any method is free of charge

Equipment available:
This includes a fume hood, 5 biohazard Class 2 hoods (4 ft wide), 1 biohazard hood vented to the hospital exhaust for use with respiratory pathogens, 1 Epics Profile II flow cytometer with 3 filters to accommodate 3 color analysis, 1 Q-Prep machine for processing samples for cytometry, 1 ultracentifuge, 10 standing centrifuges, 4 microcentrifuges (1 refrigerated), 6 water baths (1 New Brunswick shaking bath), 6 balances, 7 refrigerators, 11 freezers (5, -700 C; 2, -1350 C; 4, -200 C), 1 liquid nitrogen tank, 4 CO2 incubators, 1 spectrophotometer, 1 phase tissue culture microscope, 1 phase fluorescence microscope, 1 Betaplate scintillation counter with cell harvester,1 microtome, 2 ELISA readers, 1 UV transilluminator with camera apparatus, and 2 PCR devices (1 Perkin Elmer 48, 1 Perkin Elmer 9600), 1 Chiron b-DNA apparatus, 1 Murex LIPA processor, a semi-automated DNA sequencing system (Visible Genetics) 4 vertical (including 1 sequencing) and 3 horizontal gel electrophoresis chambers, with 3 power sources, several liquid chromatography columns with a fraction collector, 3 dot blot and 1 slot blot vacuum apparatuses, 1 microwave, 1 camera setup with UV light box, one ABI PRISM 7700 Sequence Detection system capable of 96 samples/run with data analysis time <1 minute.