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Developmental Core - Core B
In keeping with the mission of the CFAR, the Developmental Core aims:
Flow Cytometry Core - Core D
The CFAR Flow Cytometry Core provides an array of innovative flow measurements in addition
to the usual 3-color surface phenotyping of lymphocytes. Its staff is available for training
and consultation on these methods.
Immunology Core - Core E
The CFAR Immunology Core Facility provides - A range of immunologic assays - Testing of
polyclonal sera as well as human, primate and rodent anti-HIV mAbs for neutralizing activity
against a wide range of HIV primary isolates and for their ability to bind to intact virions and
to infected cells. - Provision of human anti-HIV mAbs. Frozen stocks of more than 70
mycoplasma-free cell lines producing human anti-HIV mAbs are maintained in the Core.
Virology Core - Core F
The CFAR Virology Core consists of 3 units. One concentrates on bulk and quantitative HIV
culture, measurement, resistance testing, phenotype analysis , and genotype analysis using the
heteroduplex mobility assay and sequencing. The second quantitates HIV RNA in plasma or culture
fluids by the Roche RT-PCR method. The third is a standard, licensed virology lab which detects
the presence of herpes viruses (HSV, CMV, HHV6, VZV, and EBV) by culture and/or PCR, as well as
other viruses such as HCV and JC by PCR.